"Each time we lost activity during purification of any of the components we were characterizing, we "returned" to the chromatographical column fractions and tried to reconstitute it via complementation:"
"When the radio-labeled protein was incubated in crude reticulocyte Fraction II in the presence of ATP, we observed a dramatic increase in its molecular weight: it now migrated as a sharp peak in the void volume of the gel filtration chromatographical column."
"You may hear them mention, as the dramatic music crescendos and your favorite stars pretend to be concerned, a chromatographical process."